dnastar

Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.

方法将特异性引物从S85 46病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。

Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.

方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。

Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.

方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。

Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.

方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。

The amino acid sequences were deduced and the two strains were aligned with other dengue virus type 4 strains using DNASTAR.

利用DNASTAR软件预测其氨基酸序列并与其他国内外登革4型病毒株序列比较；